Salinity affects leaf physiologic and biochemical traits of Vetiver grass (Chrysopogon zizanioides L.)

Document Type : Research Paper

Authors

1 M.Sc Student, Nature Engineering Department, Faculty of Natural Resources and Environment, Malayer University

2 Assistant Professor, Nature Engineering Department, Faculty of Natural Resources and Environment, Malayer University

Abstract

Salinity is the second most important abiotic stress in the world, especially in Iran. The research dedicated to reveal the responses of the some physiological and biochemical parameters (proline content, carbohydrates, total protein and peroxides enzymes) of Chrysopogon zizanioides to soil salinity in vitro. Prior to salinity application, the plant seedlings placed in greenhouse for adaptation purpose. To apply soil salinity stress on Chrysopogon zizanioides, sodium chloride solution was applied in 6 different concentrations in the period of 4 days. All the measurements, including leaf proline content, soluble and non-soluble sugar, protein content, and peroxidase enzyme, were measured after 2 month of salinity treatment application. The results indicated that salinity stress could have a significant increasing effects on proline content, soluble sugar and non-soluble one of leaves (p≤0.05). Leaf total protein content and peroxidase enzyme are also significantly affected by salinity stress, showing an increasing trend up to 32 ds/m following by a decreasing trend at 44 ds/m. According to physiologic and biochemical parameters, the results confirms high tolerance of Vetiver grass against 32 ds/m salinity in short term.

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Main Subjects

References

Abeles, F., and Biles, C. 1991. Characterization of peroxidases in lignifying peach fruit endocarp. Plant Physiology. 95, 269-273.
Acir, N., and Gunal, H. 2020.Soil quality of a cropland and adjacent natural grassland in an arid region. Carpathian Journal of Earth and Environmental Sciences. 15 (2), 275- 288.
Ahmadi Beni, M., Niknahad Gharmakher, H., Sadat Azimi, M., and Maramaei, M. 2014. Investigation of Forage Quality of Vetiveria zizanioides in Semi-Steppe Region of Maravehtappeh, Golestan Province, Iran. Journal of Rangeland Science. 4(4), 287-297.
Ahmadi Beni, M., Niknahad Gharmakher, H., Maramaee, M., and Sadat Azimi, M. 2016. Effects of planting Vetiver grass (Chrysopogon zizanioides) on some soil physico-chemical characteristics (A case study: Kechik station, Maraveh tapeh, Northen Iran). Rangeland. 9(3), 268-280.
Al-Sammarraie, O.N. 2020. Alsharafa, K.Y., Al-limoun, M.O., Khleifat, Kh.M., Al-Sarayreh, S.A. Al-Shuneigat, J.M. and Kalaji, H.M. Effect of various abiotic stressors on some biochemical indices of Lepidium sativum plants. Scientific Reports. 10, 21131.
Amira, M.S., and Qados, A. 2010. Effect of salt stress on plant growth and metabolism of bean plant Vicia faba (L.). Journal of the Saudi Society of Agricultural Sciences. 10, 10-16.
Ashraf, M., and Foola, M.R. 2005. Pre sowing seed treatment-ashotgun approach to improve germination, plant growth, and crop yield under saline and non-saline conditions. Advances in Agronomy. 88, 223-265.
Ashraf, M., and Haris, P.J. 2004. Potential biochemical indicator of salinity tolerance in plants, Plant Science. 166, 3-16.
Awatif, A.M., Kamal, H.E.M., and Wael Fathy, S.G.H. 2013. Effect of salinity stress on Viciafaba productivity with respect to ascorbic acid treatment. Iranian Journal of Plant Physiology. 3, 725-736.
Bates, L.S. Walderd, R.P., and Teare, I.D. 1973. Rapid determination of free proline for water-stress studies. Plant and Soil. 39(1), 205-207.
Bradford, M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of proteinutilization the principle of protein-dye binding. Analytical Biochemistry. 72, 348-254.
Chitra, T., Jayashree, S., Rathinamala, J., and Suganya, A. 2014. Preliminary Studies In Vitro Regeneration of Vetiveria Zizanioides Through Meristem Tip Culture. Journal of Bio Innovation. 3(4), 189-196.
Dasgupta, N., Nandy, P., Tiwari, C., and Das, S. 2010. Salinity-imposed changes of some isozymes and total leaf protein expression in five mangroves from two different habitats. Journal of Plant Interactions. 5(3), 211-221.
Deifel, K.S., Kopitke, P.M., and Menzies, N.W. 2006. Growth response of various perennial grasses to increasing salinity. Journal of Plant Nutrition. 29, 1573-1584.
Dell’Aversana, E., Hessini, K., Ferchichi, S., Fusco, G.M., Woodrow, P., Ciarmiello, L.F., Abdelly, C., and Carillo, P. 2021. Salinity Duration Differently Modulates Physiological Parameters and Metabolites Profile in Roots of Two Contrasting Barley Genotypes. Plants. 10(2), 307.
Dubey, R.S., and Rani, M. 1999. Influence of NaCl salinity on growth and metabolic status of proteins and amino acids in rice seedling. Journal of Agronomy. 162, 97-106.
Dubois, M., and Gille, K.A., Hamilton, J.K., Rebers, P.T., Smith, F. 1956. Colorimetric method for determination of sugars and related substance. Analytical Chemistry. 28, 350-356.
El Moukhtari, A., Cabassa-Hourton, C., Farissi, M., and Savouré, A. 2020. How does proline treatment promote salt stress tolerance during crop plant development?. Frontiers in Plant Science. 11, 1127.
Farhoudi, R., Modhej, A., and Afrous, A. 2015. Effect of salt stress on physiological and morphological parameters of rape seed cultivars. Journal of Scientific Research and Development. 2 (5), 111-117.
Formela-Luboińska, M., Chadzinikolau, T., Drzewiecka, K., Jeleń, H., Bocianowski, J., Kęsy, J., Labudda, M., Jeandet, P., and Morkunas, I. 2020. The role of sugars in the regulation of the level of endogenous signaling molecules during defense response of yellow lupine to Fusarium oxysporum. International journal of molecular sciences. 21(11), 4133.
Ferchichi, S., Hessini, K., Dell’Aversana, E., D’Amelia, L., Woodrow, P., Ciarmiello, L.F., Fuggi, A., and Carillo, P. 2018.Hordeum vulgare and Hordeum maritimum respond to extended salinity stress displaying different temporal accumulation pattern of metabolites. Functional Plant Biology. 45, 1096–1109.
Gulen, H., Turhan, E., and Eris, A. 2006. Changes in peroxidase activities and soluble proteins in strawberry varieties under salt-stress. Acta Physiologiae Plantarum. 28, 109–116.
Habibi, V., Ahmadi, H., Jafari, M., and Moeini, A. 2020. Quantitative assessment of soil salinity using remote sensing data based on the artificial neural network, case study: Sharif Abad Plain, Central Iran. Modeling Earth Systems and Environment. 1-11.
Hurkman W.J., Fornari, C.S., and Tanaka, C.K. 1989. A comparison of the effect of salt on polypeptide and translatable mRNA in roots of a salt tolerant and salt sensitive cultivar of barley. Plant Physiology. 90, 1444–1456.
Kachout, S., Mansoura, B., Jaffel, K., Leclerc, J.C., Rejeb, M.N., and Ouerghi, Z. 2009. The effects of salinity on the growth of the halophyte Atriplex hortensis (Chenopodiaceae).  Applied Ecology and Environmental Research. 7, 319-332.
Kafi, M., Stewart, W.S., and Borland, A.M. 2003. Carbohydrate and Proline Contents in Leaves, Roots, and Apices of Salt-Tolerant and Salt-Sensitive Wheat Cultivars1. Russian Journal of Plant Physiology. 50, 155–162.
Karimi, H., and Yusef-Zadeh, H. 2013. The effect of salinity level on the Morphological and Physiological Traits of Two Grape (Vitisvinifera L.) Cultivars. International Journal of Agronomy and Plant Production. 4(5), 1108-1117.
Klomjek, P., and Nitisoravut, S. 2005. Constructed treatment wetland: a study of eight plant species under saline conditions. Chemosphere. 58, 585-593.
Kuznetsov, V.I., and Shevykova, N.I. 1999. Proline under stress: Biological role, metabolism, and regulation. Russian Journal of Plant Physiology. 46, 274-287.
Kumar, V., Shiram, V., Jawali, N. and Shitole, M.G. 2007. Differential response of indica rice genotypes to NaCl stress in relation to physiological and biochemical parameters. Archives of Agronomy and Soil Science. 53(2), 581-592.
Levitt, J. 1980. Responses of plant to environmental stresses, Volume 1: Chilling, Freezing, and High Temperature Stresses. Academic Press. 
Liu, W. G., Liu, J. X., Yao, M. L., and Ma, Q. F. 2016. Salt tolerance of a wild ecotype of vetiver grass (Vetiveria zizanioides L.) in southern China. Botanical studies. 57(1), 27.
Maffei, M.E. 2002. Introduction to the genus Vetiveria. In: Maffei, M. (Ed.), Vetiveria the Genus Vetiveria. Taylor & Francis Press. 1-18.
Mane, A.V., Karadge, B.A., Suroshi, V.N., and Samant, J.S. 2013. Changes in inorganic     constituents in the leaves of Vetiveria zizanioides (L.) Nash under the influence of NaCl salinity. Octa Journal of Environmental Research. 1(4), 336-345.
Mane, A.V., Saratale, G.D., Karadg, B.A., and Samant, J.S. 2011. Studies on the effects of salinity on growth, polyphenol content and photosynthetic response in Vetiveria zizanioides (L.) Nash. Emirates Journal of Food and Agriculture. 23 (1), 59-70.
Mansour, M.M.F. 2000. Nitrogen containing compounds and adaptation of plants to salinity stress. Biologia Plantarum. 43, 491–500.
Moghaddam, M., Farhadi, N., Panjtandoust, M., and Ghanati, F. 2020. Seed germination, antioxidant enzymes activity and proline content in medicinal plant Tagetes minuta under salinity stress. Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology. 154, 835-842.
Moosikapala, L., and Te-chato, S. 2010. Application of in vitro conservation in Vetiveria zizanioides Nash. Journal of Agricultural Science and Technology. 6(2), 401-407.
Morkunas, I., and Ratajczak, L. 2014. The role of sugar signaling in plant defense responses against fungal pathogens. Acta Physiologiae Plantarum. 36, 1607–1619.
Munns, R. 2003. Comparative physiology of salt and water stress. Plant, Cell and Environment. 25, 239-250.
Munns R., and Tester, M. 2008. Mechanisms of salinity tolerance. The Annual Review of Plant Biology. 59, 651–681.
Nelson, N. 1994. A Photometric adaptation of the Somogyi method for the determination of glucose. Journal of Biological Chemistry. 153, 374-380.
Panitnok K., Thongpae, S., Sarobol, E., Chaisri, S., Ngamprasitthi, S., Pothisoong, T., Chaisri, P., Changlek, P., Thongluang, P., and Boonsri, N. 2013. Effects of Vetiver Grass and Green Manure Management on Properties of Map Bon, Coarse-Loamy Variant Soil. Communications in Soil Science and Plant Analysis. 44, 158-165.
Pareek A., Singla, S.L., and Grover, A. 1997. Salt responsive proteins/genes in crop plants, in: P.K. Jaiwal, R.P. Singh, A. Gulati (Eds.), Strategies for Improving Salt Tolerance in Higher Plants, Oxford and IBH Publication Co., New Delhi. 365–391.
Pellegrini, E., Forlani, G., Boscutti, F., and Casolo, V. 2020. Evidence of non-structural carbohydrates-mediated response to flooding and salinity in Limonium narbonense and Salicornia fruticosa. Aquatic Botany. 166, 103265.
Prasad, A., Chand, S., Kumar, S., Chattopadhyay, A., and Patra, D. D. 2014. Heavy Metals Affect Yield, Essential Oil Compound, and Rhizosphere Microflora of Vetiver (Vetiveria zizanioides Linn. nash) Grass. Communications in Soil Science and Plant Analysis. 45, 1511-1522.
Qadir, M., Qureshi, A., and Cheraghi, S. 2008. Extent and characterisation of salt-affected soils in Iran and strategies for their amelioration and management. Land Degradation & Development. 19, 214 - 227.
Sabbagh, E., Lakzayi, M., Keshtehgar, A., and Rigi, K. 2014. The effect of salt stress on respiration, PSII function, chlorophyll, carbohydrate and nitrogen content in crop plants. International Journal of Farming and Allied Sciences. 9, 988-993.
Singh, N.K., Bracken, C.A., Hasegawa, P.M., Handa, A.K., Buckel, S., Hermodson, M.A., Pfankoch, F., Regnier, F.E., and Bressan, R.A. 1987. Characterization of osmotin. A thaumatin-like protein associated with osmotic adjustment in plant cells, Plant Physiology. 85, 529–536.
Sohrabi, N., Tajabadipour, A., Motamed, N., and Seyedi, M. 2011. A Change in Leaves Protein Pattern of Some Pistachio Cultivars under Salinity Condition. International Journal of Nuts and Related Sciences. 2 (4), 67-74.
Stuart, J.R., Tester, M., Gaxiola, R.A., and Flowers, T.J. 2012. Plants of saline environments. Access Science, McGraw-Hill Education, Pennsylvania 
Truong, P.N. 2004. Vetiver grass technology for mine tailings rehabilitation. Ground and water bioengineering for erosion control and slope stabilization. 379-389.
Turhan, E., Gulen, H., and Eris, A. 2008. The activity of antioxidative enzymes in three strawberry cultivars related to salt-stress tolerance. Acta Physiologiae Plantarum. 30(2), 201-208.
Vendruscolo, E.C.G., Schuster, I., Pilegg, M., Scapim, C.A., Molinari, H.B., Marur, C.J., and Vieira, L.G. 2007. Stress-induced synthesis of proline confers tolerance to water deficit in transgenic wheat. Journal of Plant Physiology. 164(10), 1367-1376.
Vidal, J., Echevarria, C., Bakrim, N., Arrio, M., Pierre, J.N., Cretin, C., and Gadal, P. 1990. Regulation of phosphoenolypyruvate carboxylase in sorghum leaves: study of the phosphorylation process of the enzyme. In Current topics in plant biochemistry and physiology: Proceedings of the Plant Biochemistry and Physiology Symposium held at the University of Missouri, Columbia (USA). 18-25.
Zaki, R.N., and Radwan, T.E. 2011. Improving wheat grain yield and its quality under salinity conditions at a newly reclaimed soil by using different organic sources as soil or foliar applications. Journal of Applied Sciences Research. 7, 42-55.
Zhou, Q., and Yu, B.J. 2009. Accumulation of inorganic and organic osmolytes and their role in osmotic adjustment in NaCl-stressed vetiver grass seedlings. Russian Journal of Plant Physiology. 56, 678–685.
Environmental Resources Research
Volume 11, Issue 1
July 2023
Pages 77-82

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